Sehlopha sa bo-ramahlale ba Spain se khothalelitse ho ntlafatsa le ho potlakisa ho nchafatsoa ha sebete se senyehileng ho litoeba, moo ho ka lebisang kalafong e ncha ea ho phekola lefu la sebete.
Ka ho khetheha, sehlopha se etelletsoeng pele ke Juan Carlos Izpisua, oa Setsi sa Salk (USA) le UCAM, se khonne ho nchafatsa lisele tsa hydration ka mokhoa o itseng, ho li lumella ho nchafatsa lisele tse senyehileng kapele.
Liphetho, tse hatisitsoeng koranteng ea Cell Reports, li senola hore tšebeliso ea limolek'hule tsa reprogramming e ntlafatsa kholo ea lisele le ho tsosolosoa ha lisele tsa sebete ho litoeba.
Estrella Núñez le Rubén Rabadán, bangoli-'moho ba sengoloa, ka laboratoring ea 'Izpisua Belmonte - UCAM HiTech
Izpisua, ea phaellang ka hore o lumela hore ka letsatsi le leng, "mekhoa e kang ena ea ho tšoaroa ha sebete e ka hlahisa "liphello tsa rona li ka lebisa ho nts'etsopele ea mekhoa e mecha ea phekolo ea ho phekola tšoaetso, kankere, mafu a sebete a lefutso kapa mafu a metabolism a kang hepatic steatosis." ka botlalo”.
Liphoofolo tse anyesang ha li tsosolose litho tsa tsona hantle joaloka liphoofolo tse ling tse phelang lesapo la mokokotlo, tse kang litlhapi kapa li-salamanders.
Bangoli ba mosebetsi ona ba kile ba bontša kamoo tšebeliso ea limolek'hule tse 'nè tsa reprogramming cell -Oct-3/4, Sox2, Klf4 le c-Myc, eo hape e bitsoang' Yamanaka factor ', e ka liehisang botsofali le ho ntlafatsa bokhoni ba ho tsosolosoa. ea mesifa ea litoeba.
Boikemisetso ba rona bo ka lebisa ho nts'etsopele ea liphekolo tse ncha tsa ho phekola tšoaetso, mofets'e, mafu a lefutso a sebete kapa mafu a metabolic a kang hepatic steatosis.
Phuputsong ea hona joale, bafuputsi ba sebelisitse lintlha tsa Yamanaka ho eketsa tšenyo ea bohloeki le ho ntlafatsa ts'ebetso ea bohloeki, kahoo ba lelefatsa tšireletso ea litoeba ka nako. Ts'ebetso ea ho hlophisa bocha e sebelisoa ke ho fetolela lisele tse shoeleng tsa sebete ho ba lisele tse 'nyane', moo kholo le ts'ebetso ea lisele li ratoang.
Bothata boo bafuputsi ba bangata lefapheng lena ba tobanang le bona ke mokhoa oa ho laola polelo ea lintlha tsa Yamanaka ho nchafatsa lisele le ho ntlafatsa ts'ebetso ea tsona, kaha tse ling tsa limolek'hule tsena li ka laola kholo ea lisele, joalo ka mofets'e. Ka thuso ea Izpisua, bothata bona bo rarollotsoe ka ho sebelisa lintlha tsa Yamanaka ka nako e khutšoanyane.
Kalafo e ile ea fuoa litoeba tse leketlileng ka letsatsi le le leng feela ”- ho hlalosa Estrella Núñez, sengoli-'moho le sengoloa le Motlatsi oa Rector bakeng sa Lipatlisiso ho UCAM - a shebile ka hloko hore na lisele li arotsoe joang ha nako e ntse e feta. E kenyelelitsoe ka mor'a likhoeli tse robong, hoo e ka bang karolo ea boraro ea bophelo ba litoeba, ha ho le e 'ngoe ea tsona e neng e e-na le lihlahala."
Lintlha tsa Yamanaka ehlile ke sabole e sehang ka nģa tse peli, ho bolela mongoli oa pele oa pampiri, Tomohaki Hisida. "Ka lehlakoreng le leng, ba na le monyetla oa ho ntlafatsa ho nchafatsa lisele tse senyehileng tsa sebete, 'me ka lehlakoreng le leng, ba na le tšitiso ea hore ba ka hlahisa lihlahala. Fumana hore protocol e ncha ea nako e khutšoanyane ea induction e na le liphello tse ntle, empa eseng e mpe, ke ea bohlokoa: ho ntlafatsa ho tsosolosoa ha sebete 'me u se ke ua baka kankere.
O fumana hore protocol e ncha ea nako e khutšoanyane ea induction e na le liphello tse ntle, empa eseng tse mpe, ke ea bohlokoa: e ntlafatsa ho tsosolosoa ha sebete 'me ha e bake kankere.
Liphetho li bonts'a tšibollo ea bobeli ha ho ithutoa mokhoa ona oa ho hlophisa bocha litsong tsa lisele ka laboratoring: Lefutso le bitsoang Top2a, le amehang ho lokisoeng bocha ha lisele tsa sebete, le sebetsa haholo ka letsatsi le leng kamora kalafo ea nako e khuts'oane le lintlha tsa kholo. Top2a e kenyelletsa topoisomerase 2a, e leng enzyme e notlotseng senotlolo se ka morao sa DNA e le hore e khone ho hlahisa liphatsa tsa lefutso.
"Ha re thibela lefutso lena, litekanyetso tsa topoisomerase 2a li ile tsa fokotseha 'me tekanyo ea ho tsosolosoa ha lisele e fokotsehile ka makhetlo a 40, e leng se ileng sa etsa hore lisele tse ngata tse nyenyane li fokotsehe," ho bolela Rubén Rabadán, mongoli-'moho oa mosebetsi le mofuputsi oa postdoctoral UCAM. . Karolo e nepahetseng ea lefutso la Top2a ts'ebetsong ena e ntse e ithutoa."
Izpisua Belmonte o re: "Ho sa ntsane ho e-na le mosebetsi o mongata o lokelang ho etsoa pele re ka utloisisa ka botlalo motheo oa limolek'hule tse thehiloeng ho nchafatso ea lisele," ho bolela Izpisua Belmonte, e leng ntho ea bohlokoa e le hore re khone ho etsa mekhoa ea phekolo ea meriana ho fetola liphello tsa batho. mafu.”
